STAT5A antibody (Tyr694)

Catalog No. ABIN400812

Product Details anti-STAT5A Antibody

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Binding Specificity: Tyr694

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This phospho-specific monoclonal antibody reacts with Stat5a pTyr694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.

Cross-Reactivity (Details): Species reactivity (expected):Human and Rat (100 %).
Species reactivity (tested):Mouse.

Characteristics: Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A

Purification: Protein A Chromatography from concentrated tissue culture supernate.

Immunogen: Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.

Clone: 5F6-F1

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/20,000. Western blot: 1/500-1/2,000 (Expect a band approximately 91 kDa in size corresponding tophosphorylated Stat5α protein in the appropriate cell lysate or extract). Immunohistochemistry (20-40 μg/mL). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. For low volume of reagent (25 μl), to minimizeloss of volume dilute 1/10 by adding 225 μl of the buffer stated abovedirectly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at thebottom of the vial. Use this intermediate dilution when calculating final dilutions asrecommended below. Store the vial at -20 °C or below after dilution. Avoid cycles of freezing and thawing.
Shelf life: One year from despatch (3 months from date of opening).

Expiry Date: 12 months

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5 / STAT5A (STAT5A Products)

Background: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A

Gene ID: 20850

UniProt: P42230

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation