CD34 antibody
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- Target See all CD34 Antibodies
- CD34
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Reactivity
- Human, Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD34 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Specificity
- Recognizes the Mouse CD34 cell surface glycoprotein. Mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.
- Cross-Reactivity (Details)
- Species reactivity (tested):Mouse.
- Characteristics
- Synonyms: Hematopoietic progenitor cell marker, Hematopoietic progenitor cell antigen CD34
- Purification
- Protein G Chromatography.
- Clone
- MEC 14-7
- Isotype
- IgG2a
- Top Product
- Discover our top product CD34 Primary Antibody
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- Application Notes
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Immunoprecipitation: 2-10 μg/mL. Flow Cytometry: Use 10 μL of 1/25-1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one monthor (Add 40-50% Sterile Glycerol) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- CD34
- Alternative Name
- CD34 (CD34 Products)
- Background
- The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
- Gene ID
- 12490
- UniProt
- Q64314
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