RFP antibody

Catalog No. ABIN1573925

Product Details anti-RFP Antibody

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: RFP-tag Antibody, pAb, Rabbit

Sequence: VNGHEFEIEGEGEGR

Specificity: Rabbit Anti-RFP-tag Polyclonal Antibody specifically reacts with fusion proteins containing RFP-tags. The antibody does not react with GFP or GFP-tagged proteins.

Cross-Reactivity (Details): RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. The antibody does not react with GFP or GFP-tagged proteins.

Purification: Immunoaffinity chromatography

Immunogen: RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-RFP antibody (ABIN1573925)

Alvarez-Guaita, Vilà de Muga, Owen, Williamson, Magenau, García-Melero, Reverter, Hoque, Cairns, Cornely, Tebar, Grewal, Gaus, Ayala-Sanmartín, Enrich, Rentero: "Evidence for annexin A6-dependent plasma membrane remodelling of lipid domains." in: British journal of pharmacology, Vol. 172, Issue 7, pp. 1677-90, (2015) (PubMed).

Yang, Shen, Garre, Hao, Krumlinde, Cvijovi?, Arens, Nyström, Liu, Sunnerhagen: "Stress granule-defective mutants deregulate stress responsive transcripts." in: PLoS genetics, Vol. 10, Issue 11, pp. e1004763, (2014) (PubMed).

Devers, Teply, Reinert, Gaude, Krajinski: "An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula." in: BMC plant biology, Vol. 13, pp. 82, (2013) (PubMed).

Roberts, Baines: "Myosin Va enhances secretion of herpes simplex virus 1 virions and cell surface expression of viral glycoproteins." in: Journal of virology, Vol. 84, Issue 19, pp. 9889-96, (2010) (PubMed).

Nilsson, Sunnerhagen: "Cellular stress induces cytoplasmic RNA granules in fission yeast." in: RNA (New York, N.Y.), Vol. 17, Issue 1, pp. 120-33, (2010) (PubMed).

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP-Tag (RFP Products)

Background: Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.