HBxAg antibody

Catalog No. ABIN393198

Product Details anti-HBxAg Antibody

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Reactivity: Hepatitis B Virus (HBV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBxAg antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Specificity: Recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.

Purification: Affinity purified

Immunogen: HB-Xag-Protein A Fusion protein.

Type of Immunogen: Fusion protein

Clone: 3F6-G10

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:100 - 1:500)

Usage: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Hepatitis B Virus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for HBxAg

Target: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Alternative Name: Hepatitis B X Antigen (HBxAg) (HBxAg Products)

Target Type: Viral Protein