PPM1D antibody (C-Term)

Catalog No. ABIN392853

Product Details anti-PPM1D Antibody

Target: PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))

Binding Specificity: AA 571-602, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1D antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PPM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-602 amino acids from the C-terminal region of human PPM1D.

Clone: RB6018

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-PPM1D antibody (ABIN392853)

Park, Song, Kim, Han, Seol, Jang, Choi: "p53-Independent expression of wild-type p53-induced phosphatase 1 (Wip1) in methylmethane sulfonate-treated cancer cell lines and human tumors." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 896-904, (2012) (PubMed).

Song, Han, Sabapathy, Lee, Yu, Choi: "Expression of a homeostatic regulator, Wip1 (wild-type p53-induced phosphatase), is temporally induced by c-Jun and p53 in response to UV irradiation." in: The Journal of biological chemistry, Vol. 285, Issue 12, pp. 9067-76, (2010) (PubMed).

Moon, Lin, Zhang, Nguyen, Darlington, Waldman, Lu, Donehower: "Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair." in: The Journal of biological chemistry, Vol. 285, Issue 17, pp. 12935-47, (2010) (PubMed).

Zhang, Wan, Mlotshwa, Vance, Berger, Chen, Lu: "Oncogenic Wip1 phosphatase is inhibited by miR-16 in the DNA damage signaling pathway." in: Cancer research, Vol. 70, Issue 18, pp. 7176-86, (2010) (PubMed).

Nguyen, Slattery, Moon, Darlington, Lu, Donehower: "The oncogenic phosphatase WIP1 negatively regulates nucleotide excision repair." in: DNA repair, Vol. 9, Issue 7, pp. 813-23, (2010) (PubMed).

Chock, Allison, Elshamy: "BRCA1-IRIS overexpression abrogates UV-induced p38MAPK/p53 and promotes proliferation of damaged cells." in: Oncogene, Vol. 29, Issue 38, pp. 5274-85, (2010) (PubMed).

Zhang, Lin, Guo, Yang, Jones, Jochemsen, Lu: "Phosphorylation and degradation of MdmX is inhibited by Wip1 phosphatase in the DNA damage response." in: Cancer research, Vol. 69, Issue 20, pp. 7960-8, (2009) (PubMed).

Han, Yu, Song, Park, Jang, Choi: "The estrogen receptor alpha pathway induces oncogenic Wip1 phosphatase gene expression." in: Molecular cancer research : MCR, Vol. 7, Issue 5, pp. 713-23, (2009) (PubMed).

Target Details for PPM1D

Target: PPM1D (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D (PPM1D))

Alternative Name: PPM1D (PPM1D Products)

Synonyms: PP2C-DELTA antibody, WIP1 antibody, AV338790 antibody, Wip1 antibody, zgc:103518 antibody, ppm1d antibody, wu:fj44c08 antibody, zgc:55836 antibody, zgc:77316 antibody, protein phosphatase, Mg2+/Mn2+ dependent 1D antibody, protein phosphatase 1D magnesium-dependent, delta isoform antibody, protein phosphatase, Mg2+/Mn2+ dependent, 1D antibody, protein phosphatase, Mg2+/Mn2+ dependent, 1Db antibody, protein phosphatase, Mg2+/Mn2+ dependent 1D L homeolog antibody, protein phosphatase, Mg2+/Mn2+ dependent, 1Da antibody, PPM1D antibody, Ppm1d antibody, ppm1db antibody, ppm1d.L antibody, ppm1da antibody

Background: PPM1D is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. Expression of this PPM1D gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. The PPM1D gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Molecular Weight: 66675

NCBI Accession: NP_003611

UniProt: O15297

Pathways: p53 Signaling, Cell Division Cycle