CDC25A antibody (pThr507)

Catalog No. ABIN389532

Product Details anti-CDC25A Antibody

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Binding Specificity: AA 480-514, pThr507

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Predicted Reactivity: B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.

Clone: RB7856

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:250. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Expiry Date: 6 months

References for anti-CDC25A antibody (ABIN389532)

Chou, Yen, Lee, Chen: "Pro-apoptotic role of Cdc25A: activation of cyclin B1/Cdc2 by the Cdc25A C-terminal domain." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17833-45, (2010) (PubMed).

Kasahara, Goto, Enomoto, Tomono, Kiyono, Inagaki: "14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage." in: The EMBO journal, Vol. 29, Issue 16, pp. 2802-12, (2010) (PubMed).

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A (CDC25A Products)

Synonyms: cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody

Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 59087

NCBI Accession: NP_001780, NP_963861

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase