Caspase 9 antibody (pSer196)

Catalog No. ABIN389526

Product Details anti-Caspase 9 Antibody

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Binding Specificity: pSer196

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Phospho-Caspase 9-S196 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.

Clone: RB6898

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Caspase 9 antibody (ABIN389526)

Sangawa, Shintani, Yamao, Kamoshida: "Phosphorylation status of Akt and caspase-9 in gastric and colorectal carcinomas." in: International journal of clinical and experimental pathology, Vol. 7, Issue 6, pp. 3312-7, (2014) (PubMed).

Castells, Milhas, Gandy, Thibault, Rafii, Delord, Couderc: "Microenvironment mesenchymal cells protect ovarian cancer cell lines from apoptosis by inhibiting XIAP inactivation." in: Cell death & disease, Vol. 4, pp. e887, (2014) (PubMed).

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase 9 (CASP9 Products)

Synonyms: CASP9 antibody, AI115399 antibody, APAF-3 antibody, AW493809 antibody, CASP-9 antibody, Caspase-9 antibody, ICE-LAP6 antibody, Mch6 antibody, Apaf3 antibody, Casp-9-CTD antibody, Casp9_v1 antibody, Ice-Lap6 antibody, APAF3 antibody, MCH6 antibody, PPP1R56 antibody, caspase 9 antibody, CASP9 antibody, Casp9 antibody

Background: Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1, this step is thought to be one of the earliest in the caspase activation cascade.

Molecular Weight: 46281

Gene ID: 842

NCBI Accession: NP_001220, NP_001264983, NP_127463

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity