ATG9A antibody (C-Term)

Catalog No. ABIN388528

Product Details anti-ATG9A Antibody

Target: ATG9A (ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae) (ATG9A))

Binding Specificity: AA 717-746, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG9A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717-746 amino acids from the C-terminal region of human ATG9A.

Clone: RB7505-RB7506

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG9A antibody (ABIN388528)

Yang, Liu, Goga, Kim, Yuneva, Bishop: "Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 31, pp. 13836-41, (2010) (PubMed).

Gao, Kang, Liao, Ding, Gambotto, Watkins, Liu, Stolz, Yin: "Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1371-83, (2010) (PubMed).

Target Details for ATG9A

Target: ATG9A (ATG9 Autophagy Related 9 Homolog A (S. Cerevisiae) (ATG9A))

Alternative Name: ATG9A (ATG9A Products)

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.

Molecular Weight: 94447

Gene ID: 79065

NCBI Accession: NP_001070666, NP_076990

UniProt: Q7Z3C6