LC3B antibody (cleaved)

Catalog No. ABIN388484

Product Details anti-LC3B Antibody

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Binding Specificity: AA 89-122, cleaved

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.

Clone: RB15839-RB28608

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100. IF: 1:10~50. ICC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-LC3B antibody (ABIN388484)

Ock, Park, Han, Jeong, Kim, Lee, Hahm: "Genetic ablation or pharmacologic inhibition of autophagy mitigated NSAID-associated gastric damages." in: Journal of molecular medicine (Berlin, Germany), Vol. 95, Issue 4, pp. 405-416, (2018) (PubMed).

Xu, Huai, Meng, Dong, Liu, Qi, Hu, Fan, Jin, Lv: "L-3-n-Butylphthalide Activates Akt/mTOR Signaling, Inhibits Neuronal Apoptosis and Autophagy and Improves Cognitive Impairment in Mice with Repeated Cerebral Ischemia-Reperfusion Injury." in: Neurochemical research, Vol. 42, Issue 10, pp. 2968-2981, (2018) (PubMed).

Filipczak, Thomas, Chen, Salzman, McDonald, Lin, Belinsky: "TSC2 Deficiency Unmasks a Novel Necrosis Pathway That Is Suppressed by the RIP1/RIP3/MLKL Signaling Cascade." in: Cancer research, Vol. 76, Issue 24, pp. 7130-7139, (2017) (PubMed).

Lee, Park, Hahm: "Mitigated NSAID-induced apoptotic and autophagic cell death with Smad7 overexpression." in: Journal of clinical biochemistry and nutrition, Vol. 60, Issue 1, pp. 55-62, (2017) (PubMed).

Jutten, Keulers, Schaaf, Savelkouls, Theys, Span, Vooijs, Bussink, Rouschop: "EGFR overexpressing cells and tumors are dependent on autophagy for growth and survival." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 108, Issue 3, pp. 479-83, (2013) (PubMed).

Radtke, English, Rondeau, Leib, Lippé, Desjardins: "Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy." in: Journal of virology, Vol. 87, Issue 7, pp. 3990-7, (2013) (PubMed).

Checinska, Soengas: "The gluttonous side of malignant melanoma: basic and clinical implications of macroautophagy." in: Pigment cell & melanoma research, Vol. 24, Issue 6, pp. 1116-32, (2011) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Eby, Rosenbluth, Mays, Marshall, Barton, Sinha, Johnson, Tang, Pietenpol: "ISG20L1 is a p53 family target gene that modulates genotoxic stress-induced autophagy." in: Molecular cancer, Vol. 9, pp. 95, (2010) (PubMed).

Mitroulis, Kourtzelis, Kambas, Rafail, Chrysanthopoulou, Speletas, Ritis: "Regulation of the autophagic machinery in human neutrophils." in: European journal of immunology, Vol. 40, Issue 5, pp. 1461-72, (2010) (PubMed).

English, Chemali, Duron, Rondeau, Laplante, Gingras, Alexander, Leib, Norbury, Lippé, Desjardins: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, (2009) (PubMed).

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: LC3B (MAP1LC3B Products)

Synonyms: ATG8F antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1LC3B-a antibody, Map1lc3 antibody, Mpl3 antibody, zbs559 antibody, 1010001C15Rik antibody, Atg8 antibody, LC3b antibody, zgc:56434 antibody, wu:fb60g11 antibody, map1lc3b antibody, MGC76283 antibody, MAP1LC3B antibody, microtubule associated protein 1 light chain 3 beta antibody, microtubule-associated protein 1 light chain 3 beta antibody, microtubule associated protein 1 light chain 3 beta L homeolog antibody, MAP1LC3B antibody, Map1lc3b antibody, map1lc3b antibody, map1lc3b.L antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Molecular Weight: 14688

Gene ID: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagy