MAP1LC3A antibody (N-Term)

Catalog No. ABIN388473

Product Details anti-MAP1LC3A Antibody

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.

Clone: RB7481

Isotype: IgG

Application Details

Application Notes: IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-MAP1LC3A antibody (ABIN388473)

Chiu, Chen, Chang, Chen, Hsu: "Far-infrared promotes burn wound healing by suppressing NLRP3 inflammasome caused by enhanced autophagy." in: Journal of molecular medicine (Berlin, Germany), Vol. 94, Issue 7, pp. 809-19, (2018) (PubMed).

Mbah, Overmeyer, Maltese: "Disruption of endolysosomal trafficking pathways in glioma cells by methuosis-inducing indole-based chalcones." in: Cell biology and toxicology, Vol. 33, Issue 3, pp. 263-282, (2018) (PubMed).

Manu, Chai, Teh, Zhu, Casey, Wang: "Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer." in: Molecular cancer therapeutics, Vol. 16, Issue 5, pp. 914-923, (2018) (PubMed).

Huang, Wang, Lin, Huang, Chen, Chen: "The roles of autophagy and hypoxia in human inflammatory periapical lesions." in: International endodontic journal, Vol. 51 Suppl 2, pp. e125-e145, (2018) (PubMed).

Lee, Bai, Chen, Lee, Chung, Tsai, Tsao, Kao: "Histone deacetylase inhibition of cardiac autophagy in rats on a high‑fat diet with low‑dose streptozotocin-induced type 2 diabetes mellitus." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 594-601, (2018) (PubMed).

Su, Yu, Huang, Lai: "Ubiquitination of the Cytoplasmic Domain of Influenza A Virus M2 Protein Is Crucial for Production of Infectious Virus Particles." in: Journal of virology, Vol. 92, Issue 4, (2018) (PubMed).

Sorice, Iannuccelli, Manganelli, Capozzi, Alessandri, Lococo, Garofalo, Di Franco, Bombardieri, Nerviani, Misasi, Valesini: "Autophagy generates citrullinated peptides in human synoviocytes: a possible trigger for anti-citrullinated peptide antibodies." in: Rheumatology (Oxford, England), Vol. 55, Issue 8, pp. 1374-85, (2017) (PubMed).

Chiu, Yeh, Wang, Huang, Ho, Lin, Wang et al.: "Combination of the novel histone deacetylase inhibitor YCW1 and radiation induces autophagic cell death through the downregulation of BNIP3 in triple-negative breast cancer cells in vitro and in an ..." in: Molecular cancer, Vol. 15, Issue 1, pp. 46, (2017) (PubMed).

Wang, Wang, Wei, Huang, Wu, Chen, Liu, Wang: "The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies." in: Autophagy, Vol. 12, Issue 9, pp. 1560-74, (2017) (PubMed).

Buckingham, Jarosinski, Jackson, Carpenter, Grose: "Exocytosis of Varicella-Zoster Virus Virions Involves a Convergence of Endosomal and Autophagy Pathways." in: Journal of virology, Vol. 90, Issue 19, pp. 8673-85, (2017) (PubMed).

Wan, Shi, Lou, Liu, Ma, Liang, Ma: "Ghrelin protects small intestinal epithelium against sepsis-induced injury by enhancing the autophagy of intestinal epithelial cells." in: Biomedicine & pharmacotherapy, Vol. 83, pp. 1315-1320, (2017) (PubMed).

Yu, Li, Wang, Wang, Chen, Cai, He: "Low expression of MAP1LC3B, associated with low Beclin-1, predicts lymph node metastasis and poor prognosis of gastric cancer." in: Tumour biology, Vol. 37, Issue 11, pp. 15007-15017, (2017) (PubMed).

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." in: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Sanchez, Varano, de Rozieres, Maung, Catalan, Dowling, Sejbuk, Hoefer, Kaul: "Antiretrovirals, Methamphetamine, and HIV-1 Envelope Protein gp120 Compromise Neuronal Energy Homeostasis in Association with Various Degrees of Synaptic and Neuritic Damage." in: Antimicrobial agents and chemotherapy, Vol. 60, Issue 1, pp. 168-79, (2016) (PubMed).

Mhlanga-Mutangadura, Johnson, Schnabel, Taylor, Johnson, Katz, Shelton, Lever, Giuliano, Granger, Shomper, OBrien: "A mutation in the Warburg syndrome gene, RAB3GAP1, causes a similar syndrome with polyneuropathy and neuronal vacuolation in Black Russian Terrier dogs." in: Neurobiology of disease, Vol. 86, pp. 75-85, (2016) (PubMed).

Ruiz, Rockfield, Taran, Haller, Engelman, Flores, Panina-Bordignon, Nanjundan: "Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis." in: Cell death & disease, Vol. 7, pp. e2059, (2016) (PubMed).

Tai, Chew, Tan, Lim, Soong: "Iron mitigates DMT1-mediated manganese cytotoxicity via the ASK1-JNK signaling axis: Implications of iron supplementation for manganese toxicity." in: Scientific reports, Vol. 6, pp. 21113, (2016) (PubMed).

Li, Xiong, Shi, Xiao, Qi, Meng: "IKKβ/NFκBp65 activated by interleukin-13 targets the autophagy-related genes LC3B and beclin 1 in fibroblasts co-cultured with breast cancer cells." in: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1259-1264, (2016) (PubMed).

Sansanwal, Li, Sarwal: "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 3, pp. 612-25, (2015) (PubMed).

Mitsuhashi, Shindo, Shigetomi, Miyamoto, Ubukata: "(+)-Epogymnolactam, a novel autophagy inducer from mycelial culture of Gymnopus sp." in: Phytochemistry, Vol. 114, pp. 163-7, (2015) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3 (MAP1LC3A Products)

Synonyms: ATG8E antibody, LC3 antibody, LC3A antibody, MAP1ALC3 antibody, MAP1BLC3 antibody, 1010001H21Rik antibody, 4922501H04Rik antibody, LC3a antibody, MGC69006 antibody, zgc:77094 antibody, MGC89867 antibody, MAP1LC3A antibody, map1lc3a antibody, microtubule associated protein 1 light chain 3 alpha antibody, microtubule-associated protein 1 light chain 3 alpha antibody, microtubule associated protein 1 light chain 3 alpha S homeolog antibody, MAP1LC3A antibody, Map1lc3a antibody, map1lc3a.S antibody, map1lc3a antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Molecular Weight: 14688

Gene ID: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagy