Pan SUMO antibody

Catalog No. ABIN388089

Product Details

Target: Pan SUMO

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: "This Pan SUMO antibody recognizes SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length human SUMO3."

Clone: RB04602

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References

Dunphy, Luo, McBride: "Ehrlichia chaffeensis exploits host SUMOylation pathways to mediate effector-host interactions and promote intracellular survival." in: Infection and immunity, Vol. 82, Issue 10, pp. 4154-68, (2014) (PubMed).

Sun, Hunter: "Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42071-83, (2012) (PubMed).

Lutz, Wolters-Eisfeld, Joshi, Djogo, Jakovcevski, Schachner, Kleene: "Generation and nuclear translocation of sumoylated transmembrane fragment of cell adhesion molecule L1." in: The Journal of biological chemistry, Vol. 287, Issue 21, pp. 17161-75, (2012) (PubMed).

Target Details

Target: Pan SUMO

Background: Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.

Molecular Weight: 11637

Gene ID: 6612

NCBI Accession: NP_008867

UniProt: P55854