MDM2 antibody (AA 141-176)

Catalog No. ABIN388067

Product Details anti-MDM2 Antibody

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Binding Specificity: AA 141-176

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MDM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-176 amino acids from human MDM2.

Clone: RB7981

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. IHC-P: 1:10~50. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MDM2 antibody (ABIN388067)

Fragou, Tzimagiorgis, Karageorgopoulos, Barbetakis, Lazopoulos, Papaioannou, Haitoglou, Kouidou: "Increased Δ133p53 mRNA in lung carcinoma corresponds with reduction of p21 expression." in: Molecular medicine reports, Vol. 15, Issue 4, pp. 1455-1460, (2017) (PubMed).

Lei, Velez, Kazlauskas: "Pathological signaling via platelet-derived growth factor receptor {alpha} involves chronic activation of Akt and suppression of p53." in: Molecular and cellular biology, Vol. 31, Issue 9, pp. 1788-99, (2011) (PubMed).

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2 (MDM2 Products)

Background: MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Molecular Weight: 55233

Gene ID: 4193

NCBI Accession: NP_001138811, NP_001265391, NP_002383

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway