SOX2 antibody

Catalog No. ABIN387798

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: SOX2 recombinant protein is used to produce this monoclonal antibody.

Clone: 57CT23-3-4

Isotype: IgG1

Application Details

Application Notes: IF: 1:100. IF: 1:10~50. WB: 1:200~2000. WB: 1:200~2000. WB: 1:1000. WB: 1:4000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SOX2 antibody (ABIN387798)

Chen, Ou, Song, Xian, Ouyang, Xie, Xue, Sun: "Generation of integration-free induced pluripotent stem cells (GZHMUi001-A) by reprogramming peripheral blood mononuclear cells from a 47, XXX syndrome patient." in: Stem cell research, Vol. 23, pp. 57-60, (2018) (PubMed).

Horibe, Hirohashi, Asano, Mariya, Suzuki, Takaya, Saijo, Shionoya, Kubo, Nakatsugawa, Kanaseki, Tsukahara, Watanabe, Atsuyama, Toji, Hirano, Hasegawa, Takahashi, Sato, Torigoe: "Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is a novel target of lung cancer stem-like cell immunotherapy." in: PLoS ONE, Vol. 12, Issue 3, pp. e0171460, (2017) (PubMed).

Zheng, Pang, Gu, Gao, Zhang, Pang, Liu: "Melatonin Inhibits Glioblastoma Stem-like cells through Suppression of EZH2-NOTCH1 Signaling Axis." in: International journal of biological sciences, Vol. 13, Issue 2, pp. 245-253, (2017) (PubMed).

Tilson, Haley, Triantafillu, Dozier, Langford, Gillespie, Kim: "ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132823, (2015) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2 (SOX2 Products)

Background: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Molecular Weight: 34310

Gene ID: 6657

NCBI Accession: NP_003097

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis