Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , Sep - Reported in literature , Block - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, SPRD, PE/CY5.5, PACBLU, AF488, and AF700 10 L/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
1 mL
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Preservative
Without preservative
Handling Advice
Each reagent is stable for the period shown on the bottle label if stored as directed.
CD56, an isoform of the neural cell adhesion molecule (NCAM), is a 220 kDa heavily glycosylated type I transmembrane protein. On human hematopoietic cells, its expression is restricted to NK cells and a subpopulation of T lymphocytes (known as large granular lymphocytes) which demonstrate natural killer activity. While it is clear that NCAM plays a role in homotypic adhesion of neuronal cells, the significance of CD56 expression on NK cells is not clear and remains controversial.