CD8 alpha antibody (Cy5)
-
- Target See all CD8 alpha (CD8A) Antibodies
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
-
Reactivity
- Chicken
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD8 alpha antibody is conjugated to Cy5
-
Application
- Flow Cytometry (FACS)
- Specificity
- Chicken/Turkey CD8α, Mr 34 kDa
- Characteristics
- Mouse Anti-Chicken CD8a-CY5
- Purification
- Purified
- Immunogen
- Chicken thymocytes
- Clone
- 3-298
- Isotype
- IgG2b
- Top Product
- Discover our top product CD8A Primary Antibody
-
-
- Application Notes
-
- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Sep - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and CY5 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
-
- Concentration
- 0.1 mg/mL
- Buffer
- 0.1 mg in 1.0 mL of PBS/Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
-
- Target
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- Alternative Name
- CD8a (CD8A Products)
- Background
- In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two α chains, and (ii) a heterodimer of an α chain and a β chain. Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but < 1 % of cells in the bursa and bone marrow. While the vast majority of CD8 + cells in the thymus, spleen, and blood of adult chickens express both CD8 α - and CD8 β -chains, a relatively large proportion of the CD8 + TCR- γδ cells in the spleens of embryos and young chicks express only the α -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8 + T cell populations present in mice are conserved, but there is a population of TCR- γδ CD8 αβ cells in the chicken that is not found in rodents.
- Pathways
- TCR Signaling
-