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GPI antibody (pSer185)

GPI Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN375350

Datasheet as PDF

Target See all GPI Antibodies

GPI (Glucose-6-Phosphate Isomerase (GPI))
Binding Specificity
15

10

7

7

6

5

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
pSer185
Reactivity
74

48

12

6

5

4

4

4

3

3

2

2

1
Human
Host
97

15

1
Rabbit
Compatible Secondaries
Clonality
89

24
Polyclonal
Conjugate
63

8

8

7

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This GPI antibody is un-conjugated
Application
88

43

24

16

14

14

14

9

9

8

4

3

2

2

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

Reacts with human 65 kDa GPI protein with a phosphoserine at position 185

Cross-Reactivity (Details)

Do not cross react with non-phospho GPI protein.

Purification

Antiserum

Immunogen

Synthetic peptide derived from human GPI containing a phosphoserine at position 185

Isotype

IgG

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Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC : 1/200 to 1/1 000 - WB : 1/500 to 1/5 000

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Must be reconstituted in distilled water.

Storage

4 °C/-20 °C

Storage Comment

Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date

24 months
Target

GPI (Glucose-6-Phosphate Isomerase (GPI))

Abstract

GPI Products

Target Type

Viral Protein

Background

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.

UniProt

P06744