BNIP1 antibody (Internal Region, Isoform 1)

Catalog No. ABIN375177

Product Details anti-BNIP1 Antibody

Target: BNIP1 (BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1))

Binding Specificity: Internal Region, Isoform 1

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This BNIP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to BNIP1. All reported variants represent isoform 1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-DLLRQRKTTKESLAQ, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.5 - 1.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BNIP1

Target: BNIP1 (BCL2/adenovirus E1B 19kDa Interacting Protein 1 (BNIP1))

Alternative Name: BNIP1 (BNIP1 Products)

Background: BNIPs belong to the proapoptotic BH3-only protein family. Three isoforms have been described so far. BNIP 1 is involved in the maintenance of ER-integrity. It is a member of the syntaxin 18 complex which is involved in membrane trafficking between the ER and Golgi. Four different BNIP 1 splice variants have been described so far, one of which contains a highly conserved BH3 domain. All BNIP 1 variants are able to interact with the apoptosis regulators BCL2 and BCL2L1.Synonyms: BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, NIP1, SEC20L, TRG8, Transformation-related gene 8 protein, Vesicle transport protein SEC20

Gene ID: 662

NCBI Accession: NP_001196

UniProt: Q12981