FBXO32 antibody (Internal Region, Isoform 1)

Catalog No. ABIN375094

Product Details anti-FBXO32 Antibody

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Binding Specificity: Internal Region, Isoform 1

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXO32 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is expected to recognize reported isoform 1 (FBXO32).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Bovine and Porcine.
Species reactivity (tested):Human, Mouse

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence: C-NSKTKTQYFHQEK from the internal region of F-box only protein 32 isoform 1 / FBXO32

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx.40 kDa band observed in Human and Mouse SkeletalMuscle lysates (calculated MW of 41.0 kDa according to Human NP_478136.1 and of41.0 kDa according to Mouse NP_080622.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FBXO32

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Alternative Name: FBXO32 (FBXO32 Products)

Background: Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD.Synonyms: Atrogin-1, F-box only protein 32, FBXO32, MAFbx, Muscle atrophy F-box protein

Gene ID: 114907, 9606

UniProt: Q969P5