KCNJ1 antibody (Internal Region)

Catalog No. ABIN375086

Product Details anti-KCNJ1 Antibody

Target: KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNJ1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-DQININFVVD AGNEN

Specificity: This antibody is expected to recognize reported isoforms (NP_722449.2 and NP_000211) of KCNJ1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000211.1, NP_722448.1. Genename: KCNJ1

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KCNJ1

Target: KCNJ1 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))

Alternative Name: KCNJ1 (KCNJ1 Products)

Background: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. KCNJ1 is an integral membrane protein and inward rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The KCNJ1 channel is blocked by external barium. WNK1 has been shown to inhibit KCNJ1 by enhancing its endocytosis. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure.Synonyms: ATP-regulated potassium channel ROM-K, ATP-sensitive inward rectifier potassium channel 1, KCNJ1, Kir1.1, Potassium channel, ROMK1, inwardly rectifying subfamily J member 1

Gene ID: 3758

NCBI Accession: NP_000211

UniProt: P48048