FTH1 antibody (C-Term, Heavy Chain)

Catalog No. ABIN375067

Product Details anti-FTH1 Antibody

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Binding Specificity: C-Term, Heavy Chain

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-DKHTLGDSDN ES

Specificity: This antibody recognizes FTH1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_002023.2. Genename: FTH1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.03-0.1 μg/mL. Approx.22 kDa band observed in Human Brain (Cerebellum),Liver and Placenta lysates. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-FTH1 antibody (ABIN375067)

Sammarco, Ditch, Banerjee, Grabczyk: "Ferritin L and H subunits are differentially regulated on a post-transcriptional level." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4578-87, (2008) (PubMed).

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: Ferritin Heavy Chain (FTH1) (FTH1 Products)

Background: Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy, 21 kDa) and L (light, 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.Synonyms: Cell proliferation-inducing gene 15 protein, EC=1.16.3.1, FTH, FTH-1, FTHL6, Ferritin H subunit

Molecular Weight: 21.2kDa (NP_002023.2)

Gene ID: 2495

NCBI Accession: NP_002023

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis