BAG4 antibody (Internal Region)

Catalog No. ABIN375054

This anti-BAG4 antibody is a Goat Polyclonal antibody detecting BAG4 in WB. Suitable for Human.

Quick Overview for BAG4 antibody (Internal Region) (ABIN375054)

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAG4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAG4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to SODD.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-GNATSDHPNNQDQSS, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.3 - 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BAG4

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Alternative Name: BAG4

Background: Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands. Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation. SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state. SODD (Silencer of Death Domain) is an important regulator of TNF induced apoptotic pathway. It associates with TNF-R1 to prevent constitutive activation of the signaling events downstream. It dissociates from the TNF-R1 after TNF binds TNF-R1 to allow the activation of apoptotic pathway. It is expressed ubiquitously in human tissues.Synonyms: BAG family molecular chaperone regulator 4, BAG-4, Bcl-2-associated athanogene 4, SODD, Silencer of death domains

Gene ID: 9530, 9606

UniProt: O95429

Pathways: Regulation of Actin Filament Polymerization