ABCC5 antibody (N-Term)

Catalog No. ABIN375007

Product Details anti-ABCC5 Antibody

Target: ABCC5 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: KDIDIGKEYI IP-C

Specificity: This antibody is expected to recognize both reported isoforms (NP_005679.2, NP_001018881.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_005679.2, NP_001018881.1. Genename: ABCC5

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Wetsren Blot: 0.5-1.5 μg/mL. Approx.150 kDa band observed in Human Brain (FrnotalCortex) and Mouse Brain lysates. Immunohistochemistry on Paraffin Embedded Sections: 4-6 μg/mL. In paraffin embedded Human Kidney shows membranous staining in DCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ABCC5

Target: ABCC5 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 5 (ABCC5))

Alternative Name: ABCC5 / MRP5 (ABCC5 Products)

Background: MRP5 (190-200 kDa) is closely related to MRP4, both lacking the first five membrane spanning regions. MRP5 is a GS-X multi specific organic anion pump (nucleotide analogs). MRP5 may transport DNP-GS and may be inhibited by certain inhibitors of organic anion transport (sulfinpyrazone). MRP5 may also transport organic anions with the anionic moiety of phosphate/phosphonate group, a function which provides the ability to resist against anti cancer drugs 6-MP and thioguanine as well as the anti-HIV drug PMEA.Synonyms: ATP-binding cassette sub-family C member 5, MOAT-C, Multi-specific organic anion transporter C, Multidrug resistance-associated protein 5, SMRP, pABC11

Gene ID: 10057, 9606

UniProt: O15440

Pathways: Glycosaminoglycan Metabolic Process