XBP1 antibody (Internal Region)

Catalog No. ABIN374982

Product Details anti-XBP1 Antibody

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Binding Specificity: Internal Region

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-NHSWEDTFAN ELFPQ

Specificity: This antibody is expected to recognize isoform XBP1(S) only.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide from an Internal region of Human XBP1 (NP_001073007.1) Genename: XBP1

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/4000. Western Blot: 1-3 μg/mL. Approx 38 kDa band observed in Nuclear lysates of cell line HeLa(calculated MW of 40.8 kDa according to NP_001073007.1). Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-XBP1 antibody (ABIN374982)

Yokouchi, Hiramatsu, Hayakawa, Okamura, Du, Kasai, Takano, Shitamura, Shimada, Yao, Kitamura: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." in: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, (2008) (PubMed).

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1 / TREB5 (XBP1 Products)

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.Synonyms: Tax-responsive element-binding protein 5, X-box-binding protein 1, XBP-1, XBP2

Gene ID: 7494, 9606

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response