C14orf169 + NO66 antibody (Internal Region)

Catalog No. ABIN374928

Product Details anti-C14orf169 + NO66 Antibody

Target: C14orf169 + NO66 (C14orf169) (Chromosome 14 Open Reading Frame 169 (C14orf169))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This C14orf169 + NO66 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to C14orf169 / NO66.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-GAQHSDSKDPR, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C14orf169 + NO66

Target: C14orf169 + NO66 (C14orf169) (Chromosome 14 Open Reading Frame 169 (C14orf169))

Alternative Name: NO66 (C14orf169 Products)

Background: C14orf169 is the human homolog of Xenopus xNO66. xNO66 is a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. It is a novel, ubiquitous protein, which in most cell types appears to be a constitutive nucleolar component. Immunolocalization studies have revealed that this protein is highly conserved during evolution and shows in most cells analyzed a dual localization pattern, i.e., a strong enrichment in the granular part of nucleoli and in distinct nucleoplasmic entities. Colocalizations with proteins Ki-7, HP1alpha, and PCNA, respectively, have further shown that the staining pattern of xNO66 overlaps with certain clusters of late replicating chromatin. Biochemical experiments have revealed that protein NO66 cofractionates with large preribosomal particles but is absent from cytoplasmic ribosomes. It is proposed that in addition to its role in ribosome biogenesis protein xNO66 has functions in the replication or remodeling of certain heterochromatic regions.Synonyms: C14orf169, Nucleolar protein 66, hsNO66

Gene ID: 79697

NCBI Accession: NP_078920

UniProt: Q9H6W3