KCNJ11 antibody (Internal Region, N-Term)

Catalog No. ABIN374886

Product Details anti-KCNJ11 Antibody

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Binding Specificity: Internal Region, N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNJ11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-AEDPAKPRYR ARQ

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region (near the N Terminus) of the protein sequence according to NP_000516.3 Genename: KCNJ11

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.1-0.3 μg/mL. Approx 45-48 kDa band observed in Human Skeletal Musclelysates (calculated MW of 43.8 kDa according to NP_000516.3). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Pancreas shows variablestaining across the islet of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Alternative Name: KCNJ11 (KCNJ11 Products)

Background: ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.Synonyms: ATP-sensitive inward rectifier potassium channel 11, IKATP, Inward rectifier K+ channel Kir6.2, KCNJ11, Potassium channel, inwardly rectifying subfamily J member 11

Gene ID: 9606

UniProt: Q14654

Pathways: Negative Regulation of Hormone Secretion