GALR2 antibody (Internal Region)

Catalog No. ABIN374859

Product Details anti-GALR2 Antibody

Target: GALR2 (Galanin Receptor 2 (GALR2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GALR2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to GALR2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-RYPLHSRELRTPRN, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GALR2

Target: GALR2 (Galanin Receptor 2 (GALR2))

Alternative Name: GALR2 (GALR2 Products)

Synonyms: GALNR2 antibody, mGalR antibody, GALR2 antibody, GAL2-R antibody, GALR-2 antibody, galanin receptor 2 antibody, transcription regulator. LacI family, raffinose related antibody, DNA-binding transcriptional regulator GalR antibody, galactose operon repressor antibody, galanin receptor type 2 antibody, GALR2 antibody, Galr2 antibody, LOC100037698 antibody, rafR antibody, galR2 antibody, LOC103690156 antibody

Background: Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30 amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7 transmembrane family of G protein coupled receptors. GALR2 interacts with the N terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways.GalR2 expression has been documented widely in the brain and in peripheral tissues including small intestine, heart, kidney, and liver. Localization data from rat CNS indicate high levels of expression in dorsal root ganglia, amygdala and Purkinje neurons.Synonyms: GAL2-R, GALNR2, GALR-2, Galanin receptor type 2

Gene ID: 8811

NCBI Accession: NP_003848

UniProt: O43603

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Feeding Behaviour