ROBO3 antibody (Internal Region)

Catalog No. ABIN374821

Product Details anti-ROBO3 Antibody

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ROBO3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-RRKQRKELSH YT

Specificity: This antibody detects ROBO3/RIG1 (internal).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence Genename: ROBO3

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: ROBO3 (ROBO3 Products)

Background: Roundabout proteins 1-3, known as Robo1-3, are repulsive axon guidance receptors that bind to Slit, a repellent secreted by midline glia during development. Robo3 (also known as Rig1) is important for normal axon navigation at the ventral midline of the neural tube during development and is specifically expressed by commissural axons in rodents. Loss-of-function studies in mice show that Robo3 is required to allow commissural axons to enter the floor plate and cross to the contralateral side of the spinal cord. Some isoforms of Robo3 exist.Synonyms: ROBO-3, Roundabout homolog 3, Roundabout-like protein 3

Molecular Weight: 148kDa (NP_071765.2).

Gene ID: 64221, 9606

UniProt: Q96MS0