SALL4 antibody (Internal Region)

Catalog No. ABIN374800

Product Details anti-SALL4 Antibody

Target: SALL4 (Sal-Like 4 (SALL4))

Binding Specificity: Internal Region

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SALL4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-DQSKKGKGKP QN

Specificity: This antibody detects SALL4.

Cross-Reactivity (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Rat

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_780512.2, NP_958797.2, NP_958798.2. Genename: Sall4

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Rat Spleen and Testis lysates(calculated MW of 66.0 kDa according to NP_958797.2). Additional bands of unknownidentity was also consistently observed at 28+20 kDa. These bands were successfullyblocked by incubation with the immunising peptide. We would appreciate any feedbackfrom people in the field - have any such results been reported with otherantibodies/lysates? Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SALL4

Target: SALL4 (Sal-Like 4 (SALL4))

Alternative Name: Sal-Like Protein 4 (SALL4) (SALL4 Products)

Synonyms: SALL4 antibody, drrs antibody, sall4a antibody, znf797 antibody, MGC81541 antibody, hsal4 antibody, MGC76059 antibody, cb614 antibody, sb:cb372 antibody, sb:cb614 antibody, wu:fa01g03 antibody, ik:tdsubc_2c1 antibody, xx:tdsubc_2c1 antibody, 5730441M18Rik antibody, AA407717 antibody, AL022809 antibody, AW536104 antibody, C330011P20Rik antibody, C78083 antibody, C78563 antibody, Tex20 antibody, DRRS antibody, HSAL4 antibody, ZNF797 antibody, dJ1112F19.1 antibody, spalt like transcription factor 4 antibody, sal-like 4 (Drosophila) antibody, spalt-like transcription factor 4 L homeolog antibody, spalt-like transcription factor 4 antibody, SALL4 antibody, sall4 antibody, sall4.L antibody, Sall4 antibody

Background: Sall4 is a probable transcription factor. Defects in Sall4 are the cause of Okihiro syndrome, also known as Duane radial ray syndrome (DRRS). It is a disorder characterized by the association of forearm malformations with Duane retraction syndrome. Sall4 is also involved in forelimb and heart development in miceSynonyms: Zinc finger protein SALL4

Gene ID: 99377

NCBI Accession: NP_780512

UniProt: Q8BX22

Pathways: Stem Cell Maintenance, Tube Formation