STAG2 antibody (Internal Region)

Catalog No. ABIN374783

Product Details anti-STAG2 Antibody

Target: STAG2 (Stromal Antigen 2 (STAG2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This STAG2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is expected to recognise isoform a and isoform b of STAG2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-REPKRLRPEDS, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.01 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STAG2

Target: STAG2 (Stromal Antigen 2 (STAG2))

Alternative Name: STAG2 / Stromal Antigen 2 (STAG2 Products)

Background: SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.Synonyms: Cohesin subunit SA-2, SA2, SCC3 homolog 2

Gene ID: 10735

NCBI Accession: NP_001036214

UniProt: Q8N3U4

Pathways: Stem Cell Maintenance