COL7A1 antibody (Internal Region)
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- Target See all COL7A1 Antibodies
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This COL7A1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- This antibody reacts to Collagen, type VII. This product does not work on cryostat sections of Normal Human Skin.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Human, Canine, Porcine.
Species reactivity (tested):Rat. - Purification
- Affinity chromatography
- Immunogen
- Peptide with sequence C-TVQYSDDPRTEF, from the internal region of the protein sequence
- Top Product
- Discover our top product COL7A1 Primary Antibody
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- Application Notes
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Peptide ELISA: 1/32000. Western Blot: 0.1 - 1.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))
- Alternative Name
- Collagen Type VII (COL7A1 Products)
- Background
- Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.Synonyms: COL7A1, LC collagen, Long-chain collagen
- Gene ID
- 1294
- NCBI Accession
- NP_000085
- UniProt
- Q02388
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