SECISBP2 antibody (Internal Region)

Catalog No. ABIN374739

Product Details anti-SECISBP2 Antibody

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Binding Specificity: Internal Region

Reactivity: Human, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This SECISBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-KERQERKQRL QEN

Specificity: This antibody recognizes SBP2.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Pig, Cow.
Species reactivity (tested):Human.

Purification: Affinity Chromatpography

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_076982.3.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 3 μg/mL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SECISBP2

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Alternative Name: SECISBP2 (SECISBP2 Products)

Background: SECISBP2 is an RNA binding protein that interacts with the Sec insertion sequence (SECIS) element in selenoprotein mRNAs. The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. Mutations in the SECISBP2 gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism.Synonyms: SBP2, SECIS-binding protein 2, Selenocysteine insertion sequence-binding protein 2

Gene ID: 79048

NCBI Accession: NP_076982

UniProt: Q96T21