SV2A antibody (Internal Region)

Catalog No. ABIN374729

Product Details anti-SV2A Antibody

Target: SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SV2A antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes SV2A.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Human, Canine.
Species reactivity (tested):Rat.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-TVELYPSDKRTTA, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in rat brain lysates (calculatedMW of 82.0 kDa according to NP_476558.1 (rat)). An additonal band of unknown identitywas also consistently observed at 20 kDa. This band was successfully blocked byincubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SV2A

Target: SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))

Alternative Name: SV2A (SV2A Products)

Background: SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.Synonyms: KIAA0736, Synaptic vesicle glycoprotein 2A

Gene ID: 9900

NCBI Accession: NP_055664

UniProt: Q7L0J3