AGO1 antibody (Internal Region)

Catalog No. ABIN374655

Product Details anti-AGO1 Antibody

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes Protein eIF2C1 /AGO1. This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.3-1 μg/mL. In transfected HEK293 transiently expressing Human EIF2C1bands of approx.110 kDa and 30 kDa are observed. The 110 kDa band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: EIF2C1 / AGO1 (EIF2C1 Products)

Background: Ago1 belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as cell cycle control protein through it's regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.Synonyms: Argonaute-1, Eukaryotic translation initiation factor 2C1, RNA-binding protein Q99, eIF-2C1, hAgo-1

Molecular Weight: 97.2kDa according to NP_036331.1

Gene ID: 26523

NCBI Accession: NP_036331

UniProt: Q9UL18

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization