IFT88 antibody (C-Term)

Catalog No. ABIN374643

Product Details anti-IFT88 Antibody

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT88 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-KKRIDEDDFA DEE

Specificity: This antibody is expected to recognize both reported isoforms (NP_783195.2, NP_006522.2).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, MOuse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence (NP_783195.2, NP_006522.2). Genename: IFT88

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 1 μg/mL. Immunohistochemistry on paraffin sections: 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IFT88 antibody (ABIN374643)

Madrigal-Matute, Fernandez-Garcia, Gomez-Guerrero, Lopez-Franco, Muñoz-Garcia, Egido, Blanco-Colio, Martin-Ventura: "HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis." in: Cardiovascular research, Vol. 95, Issue 1, pp. 116-23, (2012) (PubMed).

Schachner, Golderer, Sarg, Lindner, Bonaros, Mikuz, Laufer, Werner: "The amounts of alpha 1 antitrypsin protein are reduced in the vascular wall of the acutely dissected human ascending aorta." in: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, Vol. 37, Issue 3, pp. 684-90, (2011) (PubMed).

Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." in: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

Patzak, Lai, Fähling, Sendeski, Martinka, Persson, Persson: "Adenosine enhances long term the contractile response to angiotensin II in afferent arterioles." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 293, Issue 6, pp. R2232-42, (2008) (PubMed).

Target Details for IFT88

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Alternative Name: IFT88 (IFT88 Products)

Background: TTC10 is a member of the tetratrico peptide repeat (TPR) family. Mutations in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene.Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, TG737, TPR repeat protein 10, TTC10, Tetratricopeptide repeat protein 10

Gene ID: 8100

NCBI Accession: NP_006522

UniProt: Q13099

Pathways: Hedgehog Signaling, Sensory Perception of Sound