UCP1 antibody (C-Term)

Catalog No. ABIN374628

Product Details anti-UCP1 Antibody

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-EQLKRELSKS RQ

Specificity: This antibody recognizes Thermogenin (UCP1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_068605.1

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.1 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: SLC25A7 / UCP1 (UCP1 Products)

Background: The uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that are considered to be transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They are capable of returning protons pumped by the respiratory chain to the mitochondrial matrix. Uncoupling proteins currently comprise UCP1, UCP2, UCP3, UCP4, and UCP5. UCP1 is a 32 kDa protein that is active as a proton channel forming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids.Synonyms: Mitochondrial brown fat uncoupling protein 1, Solute carrier family 25 member 7, Thermogenin, UCP 1, UCP-1

Gene ID: 7350, 9606

UniProt: P25874

Pathways: Proton Transport, Brown Fat Cell Differentiation