DAZL antibody (Internal Region)

Catalog No. ABIN374590

Product Details anti-DAZL Antibody

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DAZL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-GNGPQKKSVD R

Specificity: This antibody recognizes DAZL.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide corresponding to the internal sequence of Human DAZL according to NP_001342.2 Genename: DAZL

Application Details

Application Notes: Peptide ELISA: 1/64000. Western blot: Approx 35 kDa band observed in human ovary and human testes lysates(calculated MW of 33.5 kDa according to NP_001342.2). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Mouse Testis shows cytoplasm staining ofspermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatidsbut this additional staining is not in agreement with results obtained by another antibody(Ruggiuet al, Nature.1997 Sep 4,389(6646): 73-7. PMID: 9288969). Data kindly provided by J. S. Martins, MRC, Edinburgh, UK. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Alternative Name: DAZL (DAZL Products)

Background: DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.Synonyms: DAZ homolog, DAZ-like autosomal, DAZH, DAZL, DAZL1, DAZLA, Deleted in azoospermia-like, Deleted in azoospermia-like 1, SPGY-like-autosomal, SPGYLA

Gene ID: 1618

NCBI Accession: NP_001342

UniProt: Q92904