ADH1A antibody (N-Term)

Catalog No. ABIN374506

Product Details anti-ADH1A Antibody

Target: ADH1A (Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Chimpanzee

Host: Goat

Clonality: Polyclonal

Conjugate: This ADH1A antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is expected to recognize the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of Human alcohol dehydrogenase.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.3-1.5 μg/mL. Approx 38 kDa band observed in Human Liver lysates(calculated MW of 39.8 kDa according to NP_000658.1, NP_000659.2 and 000660.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as presrevative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADH1A

Target: ADH1A (Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A))

Alternative Name: Alcohol Dehydrogenase 1A (ADH1) (ADH1A Products)

Background: ADH (Alcohol dehydrogenase) consists of a dimer of identical or nonidentical chains of three types, alpha, beta and gamma. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. There are 7 different ADH's isozymes in human: three belongs to class I: alpha, beta, and gamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.Synonyms: ADH1A, Alcohol dehydrogenase subunit alpha

Gene ID: 124, 9606

UniProt: P07327