NR1H2 antibody (N-Term)

Catalog No. ABIN374475

Product Details anti-NR1H2 Antibody

Target: NR1H2 (Nuclear Receptor Subfamily 1, Group H, Member 2 (NR1H2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NR1H2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: SSPTTSSLDT PLPGC

Specificity: This antibody is expected to be specific for LXR beta and not to cross-react with LXR alpha.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_009052.3

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/64000. Western blot: 1-3 μg/mL. Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Spleen shows nucleolarstaining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for NR1H2

Target: NR1H2 (Nuclear Receptor Subfamily 1, Group H, Member 2 (NR1H2))

Alternative Name: NR1H2 (NR1H2 Products)

Background: LXR beta is an orphan receptor which belongs to the nuclear hormone receptor family, NR1 subfamily. Ubiquitously expressed, it binds preferentially to double stranded oligonucleotide direct repeats having the consensus half site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Forms a heterodimer with RXR.Synonyms: LXRB, Liver X receptor beta, NER, Nuclear orphan receptor LXR-beta, Nuclear receptor NER, Nuclear receptor subfamily 1 group H member 2, Oxysterols receptor LXR-beta, UNR, Ubiquitously-expressed nuclear receptor

Gene ID: 7376

NCBI Accession: NP_009052

UniProt: P55055

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding