Caspase 4 antibody (Internal Region)

Catalog No. ABIN374466

Product Details anti-Caspase 4 Antibody

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CKERAEEIYP IKERN

Specificity: This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP-150649.1).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 40 kDa band observed in Human Heart lysates(calculated MW of 43.0 kDa according to NP_001216.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase-4 (CASP4 Products)

Background: Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67 % homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX

Gene ID: 837, 9606

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity