FTCD antibody (N-Term)

Catalog No. ABIN374453

Product Details anti-FTCD Antibody

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FTCD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: SQLVECVPNF SEGKNQ

Specificity: This antibody recognizes 58K Golgi protein at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Pig.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the N-Terminus of the protein sequence according to NP_006648.1, NP_996848.1. Genename: FTCD

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.5 μg/mL. Detects a 55-60 kDa band in Human Liver lysate (PredictedMolecular Weight: 58.0 kDa). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm ina selection of hepatocytes.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FTCD

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Alternative Name: 58K Golgi Protein (FTCD Products)

Background: 58K Golgi protein antibodies are excellent for use as markers for the Golgi complex. The 58K Golgi protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency, also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.Synonyms: FTCD, Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, Golgi Marker 58K, Golgi marker, LCHC1

Gene ID: 10841, 9606

UniProt: O95954