CPEB1 antibody (C-Term)

Catalog No. ABIN374439

Product Details anti-CPEB1 Antibody

Target: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Binding Specificity: C-Term

Reactivity: Human, Macaque

Host: Goat

Clonality: Polyclonal

Conjugate: This CPEB1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-PLMRNQKNRD SS

Specificity: This antibody recognizes CPEB1.

Cross-Reactivity (Details): Species reactivity (expected):Macaque.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_085097.

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL. Approx 65-70 kDa band observed in Human Brain lysates(predicted MW of 60 kDa according to NP_085097). Please note this band was always accompanied by a slightly stonger band at approx 40 kDaand further both bands could be blocked by incubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CPEB1

Target: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Alternative Name: CPEB1 (CPEB1 Products)

Background: The regulated translation of messenger RNA is essential for cell cycle progression, establishment of the body plan during early development, and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, which is one mechanism of controlling translation, is driven by CPEB1 (Cytoplasmic Polyadenylation Element Binding protein), a highly conserved, sequence specific RNA binding protein that binds to the cytoplasmic polyadenylation element (CPE) and modulates translational repression and mRNA localisation. CPEB1 can assemble bothe translational activation and repression complexes via its binding to the CPE. In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB1.Synonyms: CPE-BP1, CPE-binding protein 1, Cytoplasmic polyadenylation element-binding protein 1, h-CEBP, hCPEB-1

Gene ID: 64506

NCBI Accession: NP_001073001

UniProt: Q9BZB8

Pathways: Synaptic Membrane