RGS18 antibody (C-Term)

Catalog No. ABIN374434

Product Details anti-RGS18 Antibody

Target: RGS18 (Regulator of G-Protein Signalling 18 (RGS18))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RGS18 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CNEFQDVQSD VAIWL

Specificity: This antibody recognizes RGS18.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_570138.1

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: 1/16,000. Western Blot: 1-3 μg/mL. Approx 28 kDa band observed in Human Peripheral BloodMononucleocytes (lymphocytes) lysates (calculated MW of 27.8 kDa according toNP_570138.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RGS18

Target: RGS18 (Regulator of G-Protein Signalling 18 (RGS18))

Alternative Name: RGS18 (RGS18 Products)

Background: Regulators of G-protein signaling (RGSs) are a protein family that can act as GTPase-activating proteins for G(alphai)- and G(alphaq)-class proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination. One of these proteins, RGS18, is a 235 amino acid protein that is closely related to RGS5 (46 % identity) and approximately 30-40 % identity to other RGS proteins. This protein is expressed in platelet, leukocyte, and megakaryocyte cell lines. Engagement of TLR3 or TLR4 on monocyte-derived DCs potently down-regulates RGS18 and RGS14 without modifying other RGS proteins. A similar pattern of RGS protein expression occurs in immature bone marrow-derived mouse DCs stimulated to mature via TLR4 signaling. The changes in RGS18 and RGS1 expression are likely important for DC function.Synonyms: RGS13, Regulator of G-protein signaling 18

Gene ID: 64407

NCBI Accession: NP_570138

UniProt: Q9NS28

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling