NIR1 antibody (C-Term)

Catalog No. ABIN374424

Product Details anti-NIR1 Antibody

Target: NIR1 (PITPNM3) (PITPNM Family Member 3 (PITPNM3))

Binding Specificity: C-Term

Reactivity: Human, Dog, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NIR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-SWARGPPKFE SVP

Specificity: This antibody detects NIR1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_112497.2, NP_001159438.1. Genename: PITPNM3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: Preliminary experiments gave an approx 50 kDa band in Human Heart lysatesafter 0.1 μg/mL antibody staining. The 50 kDa band was successfully blocked by incubationwith the immunizing peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embeddedHuman Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for NIR1

Target: NIR1 (PITPNM3) (PITPNM Family Member 3 (PITPNM3))

Alternative Name: PITPNM3 (PITPNM3 Products)

Background: This gene encodes a member of a family of membrane-associated phosphatidylinositol transfer domain-containing proteins. The calcium-binding protein has phosphatidylinositol (PI) transfer activity and interacts with the protein tyrosine kinase PTK2B (also known as PYK2). The protein is homologous to a Drosophila protein that is implicated in the visual transduction pathway in flies. Mutations in this gene result in autosomal dominant cone dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.Synonyms: Membrane-associated phosphatidylinositol transfer protein 3, NIR-1, NIR1, PITPNM 3, Phosphatidylinositol transfer protein, Pyk2 N-terminal domain-interacting receptor 1, membrane-associated 3

Gene ID: 83394

NCBI Accession: NP_112497

UniProt: Q9BZ71