AKAP8 antibody (N-Term)

Catalog No. ABIN374421

Product Details anti-AKAP8 Antibody

Target: AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This AKAP8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DQGYGGYGAW SAG-C

Specificity: This antibody detects AKAP8 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_005849.1. Genename: AKAP8

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Experiments gave a band at approx 100 kDa in Human Liver lysateafter 1 μg/mL antibody staining. This band corresponds to the 90 kDa band reported inliterature (PMID: 10601332). This protein has a calculated MW of 76.0 kDa according toNP_005849.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for AKAP8

Target: AKAP8 (A Kinase (PRKA) Anchor Protein 8 (AKAP8))

Alternative Name: AKAP8 (AKAP8 Products)

Background: The A kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This antibody recognises a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.Synonyms: A-kinase anchor protein 8, A-kinase anchor protein 95 kDa, AKAP95

Gene ID: 10270

NCBI Accession: NP_005849

UniProt: O43823

Pathways: SARS-CoV-2 Protein Interactome