VDAC2 antibody (C-Term)

Catalog No. ABIN374420

Product Details anti-VDAC2 Antibody

Target: VDAC2 (Voltage-Dependent Anion Channel 2 (VDAC2))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This VDAC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-GHKVGLALEL EA

Specificity: This antibody detects VDAC-2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine (Dog), Bovine and Pig.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_003366.2 Genename: VDAC2

Application Details

Application Notes: Peptide ELISA: Antibody Limit dilution 1/64000. Western blot: 0.3-1.0 μg/mL. Approx 30 kDa band observed in Human Brain and HumanTestis lysates (calculated MW of 31.0 kDa according to NP_003366.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

References for anti-VDAC2 antibody (ABIN374420)

Vilagran, Yeste, Sancho, Casas, Rivera del Álamo, Bonet: "Relationship of sperm small heat-shock protein 10 and voltage-dependent anion channel 2 with semen freezability in boars." in: Theriogenology, Vol. 82, Issue 3, pp. 418-26, (2014) (PubMed).

Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

Okamoto, Inoue, Iwamura, Terashima, Soya, Asashima, Kuwabara: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, (2011) (PubMed).

Sroka, Voigt, Deeg, Reed, Schulz, Bhr, Kermer: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).

Target Details for VDAC2

Target: VDAC2 (Voltage-Dependent Anion Channel 2 (VDAC2))

Alternative Name: VDAC2 (VDAC2 Products)

Background: VDAC2 forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. VDAC2 interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. It consists mainly of membrane spanning sided beta sheets, and belongs to the eukaryotic mitochondrial porin family.Synonyms: Outer mitochondrial membrane protein porin 2, VDAC-2, Voltage-dependent anion-selective channel protein 2

Gene ID: 7417

NCBI Accession: NP_003366

UniProt: P45880