SIPA1 antibody (C-Term)
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- Target See all SIPA1 Antibodies
- SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SIPA1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-ASKQLGSPTA DLA
- Specificity
- This antibody detects SPA1 / SIPA1 at C-term.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from C Terminus of the protein sequence according to NP_694985.28, NP_006738.3 Genename: SIPA1
- Top Product
- Discover our top product SIPA1 Primary Antibody
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anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (AA 759-927) antibody
SIPA1 Reactivity: Human ELISA, WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (C-Term) antibodySIPA1 Reactivity: Human WB, IHC (p), IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (AA 942-970), (C-Term) antibodySIPA1 Reactivity: Human WB Host: Rabbit Polyclonal RB36710 unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (C-Term) antibodyVerified SIPA1 Reactivity: Human ELISA, WB Host: Goat Polyclonal unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) antibodySIPA1 Reactivity: Mouse ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (AA 863-912) antibodySIPA1 Reactivity: Human, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (AA 933-1042) antibodySIPA1 Reactivity: Human ELISA Host: Mouse Monoclonal 2B4 unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) (AA 763-1042) antibodySIPA1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) antibodySIPA1 Reactivity: Human WB, IP Host: Mouse Monoclonal 331-9 unconjugated
anti-Signal-Induced Proliferation-Associated 1 (SIPA1) antibodySIPA1 Reactivity: Mouse, Hamster WB, IP Host: Mouse Monoclonal 2-22-9 unconjugated
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- Application Notes
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.150 kDa band observed in Human lysates ofEBV-immortilized lymphoblastoid (calculated MW of 110 kDa according to NP_694985.28and NP_006738.2). The observed molecular weight corresponds to earlier findings inliterature with different antibodies (Kurachi et al, J Biol Chem.1997 Oct31,272(44): 28081-8, PMID: 9346962). An additional band of 55-60 kDa was consistentlyobserved, however this band was not blocked by the immunizing peptide and it istherefore a non-specific signal.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))
- Alternative Name
- SIPA1 / SPA1 (SIPA1 Products)
- Background
- Synonyms: GTPase-activating protein Spa-1, Signal-induced proliferation-associated protein 1, Sipa-1, Spa-1, p130 SPA-1
- Gene ID
- 6494
- UniProt
- Q96FS4
- Pathways
- Response to Water Deprivation
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