MPP5 antibody (N-Term)

Catalog No. ABIN374386

Product Details anti-MPP5 Antibody

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Binding Specificity: N-Term

Reactivity: Human, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MPP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: NKASPPFPLI SNAQ-C

Specificity: This antibody detects MPP5 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Gorilla, Orangutan, Gibbon, Monkey, Bovine, Dog, Horse, Pig, Rabbit.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_071919.1 Genename: MPP5

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 75 kDa band observed in Human Brain lysate (calculatedMW of 77.5 kDa according to NP_071919). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Skeletal Muscle showsstrong alternating staning of muscle fibres in longitudinal section.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MPP5

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Alternative Name: MPP5 (MPP5 Products)

Background: Members of the peripheral membrane associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ/ SH3/ GUK core and are divided into 4 subfamilies (DLG like, ZO1 like, p55 like and LIN2 like) based on their size and the presence of additional domains. MPP5 is a member of the p55 like MAGUK subfamily. The gene product of MPP5 is thought to interact with the LIN7 proteins.Synonyms: MAGUK p55 subfamily member 5

Gene ID: 64398

NCBI Accession: NP_071919

UniProt: Q8N3R9

Pathways: Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization