ATP6AP2 antibody (C-Term)

Catalog No. ABIN374363

Product Details anti-ATP6AP2 Antibody

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP6AP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SIIYRMTNQK IRMD

Specificity: This antibody detects Renin Receptor at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Cow.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005756.2 Genename: ATP6AP2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.25-1 μg/mL. Approx 37 kDa band observed in Human Kidney lysates(predicted size of approx.40Kda according to NP_005756).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ATP6AP2 antibody (ABIN374363)

Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, (2013) (PubMed).

Zhu, Yoneda, Demura, Karashima, Usukura, Yamagishi, Takeda: "Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats." in: Journal of hypertension, Vol. 27, Issue 4, pp. 800-5, (2009) (PubMed).

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Alternative Name: Renin Receptor (ATP6AP2 Products)

Background: Synonyms: ATP6AP2, ATP6IP2, ATP6M8-9, ATPase H(+)-transporting lysosomal accessory protein 2, ATPase H(+)-transporting lysosomal-interacting protein 2, CAPER, ELDF10, ER-localized type I transmembrane adaptor, Embryonic liver differentiation factor 10, HT028, MSTP009, N14F, PSEC0072, Renin/prorenin receptor, Vacuolar ATP synthase membrane sector-associated protein M8-9

Gene ID: 10159

NCBI Accession: NP_005756

UniProt: O75787

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones