Coronin 1a antibody (C-Term)

Catalog No. ABIN374350

Product Details anti-Coronin 1a Antibody

Target: Coronin 1a (CORO1A) (Coronin, Actin Binding Protein, 1A (CORO1A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Coronin 1a antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-KRLDRLEETV QAK

Specificity: This antibody detects Coronin-1A / TACO at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_009005.1 Genename: CORO1A

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.25-1 μg/mL. Approx 60 kDa band observed in Molt-4 and Jurkat lysates(predicted MW of 50 kDa according to NP_009005.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Coronin 1a

Target: Coronin 1a (CORO1A) (Coronin, Actin Binding Protein, 1A (CORO1A))

Alternative Name: Coronin-1A (CORO1A Products)

Background: Coronin 1a belongs to the WD repeat coronin family and contains 5 WD repeats. It may be an essential component of the cytoskeleton of highly motile cells, with roles both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In cell infected by mycobacteria, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. In the CNS, coronin 1A is expressed exclusively by microglial cells. Although its function is not entirely clear, it may act as a linkage protein between the actin cytoskeleton and the plasma membrane, and perhaps the extracellular matrix.Synonyms: CORO1, CORO1A, Clipin-A, Coronin-like protein, Coronin-like protein p57, TACO, Tryptophan aspartate-containing coat protein

Gene ID: 11151

NCBI Accession: NP_009005

UniProt: P31146

Pathways: Regulation of Actin Filament Polymerization