Rabenosyn 5 antibody (C-Term)

Catalog No. ABIN374348

Product Details anti-Rabenosyn 5 Antibody

Target: Rabenosyn 5 (ZFYVE20) (Zinc Finger, FYVE Domain Containing 20 (ZFYVE20))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Rabenosyn 5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-ELKHTLAKQK GGTD

Specificity: This antibody detects Rabenosyn-5 (ZFYVE20) at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_071735 Genename: ZFYVE20

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.3 - 1.5 μg/mL. Approx 110 kDa band observed in A431, Fibroblast(epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Rabenosyn 5

Target: Rabenosyn 5 (ZFYVE20) (Zinc Finger, FYVE Domain Containing 20 (ZFYVE20))

Alternative Name: Rabenosyn-5 / ZFYVE20 (ZFYVE20 Products)

Background: Synonyms: FYVE finger-containing Rab5 effector protein rabenosyn-5, Rabenosyn 5, Zinc finger FYVE domain-containing protein 20

Gene ID: 64145

NCBI Accession: NP_071735

UniProt: Q9H1K0

Pathways: Toll-Like Receptors Cascades